Title: Ultrasensitive Detection of Hepatitis C Virus DNA Subtypes Based on Cucurbituril and Graphene Oxide Nano-composite 

Author(s): Jiang, P (Jiang, Ping); Li, YF (Li, Yifei); Ju, T (Ju, Ting); Cheng, WB (Cheng, Wenbo); Xu, JH (Xu, Jianhua); Han, K (Han, Kun) 

Source: CHEMICAL RESEARCH IN CHINESE UNIVERSITIES  Volume: 36  Issue: 2  Special Issue: SI  Pages: 307-312  DOI: 10.1007/s40242-020-9111-8  Early Access Date: MAR 2020   Published: APR 2020   

Abstract: Infectious of hepatitis C viruses(HCVs) lead to hepatic fibrosis, cirrhosis even hepatoma. Developing rapid and sensitive diagnostic method for HCV is of great importance. Based on the host-and-guest interaction between cucurbit[7]uril(CB[7]) and methylene blue(MB), a CB[7]-graphene nano-composite(CB[7]-N-3-GO) is raised for the electrochemical detection of HCV DNA. The method is able to linearly detect the HCV nucleic acid in the range of 0.2-10 nmol/L with detection limit as low as 160.4 pmol/L. The proposed detection strategy is able to discriminate the 1b and 6k subtypes of HCV and has a prospective potential in the blood screen for HCV in clinical diagnosis. 

ISSN: 1005-9040 

eISSN: 2210-3171